Omega-3 and Vitamin E Supplementation Effect on Reactive Oxygen Species and Placental Vascular Endothelial Growth Factor: Pre-eclampsia Model

Abstract

BACKGROUND: Pre-eclampsia (PE) has been associated with oxidative stress and inflammation. Omega-3 fatty acids and vitamin E have beneficial function to maintain cell membrane, prevent oxidative stress, and inhibit the production of proinflammatory cytokines. The purpose of the study was to determine the effect of omega-3 and vitamin E supplement to Reactive Oxygen Species (ROS) and placental Vascular Endothelial Growth Factor (VEGF) on PE rats model. AIM: This study aimed to investigate the effect of omega-3 and vitamin E supplementation on ROS and placental VEGF. METHODS: This research has been carried out at animal house and Biomedical Laboratory of the Medical Faculty Andalas University. The design of this research was experimental study with post-test only control group design. Thirty pregnant rats were divided into five groups. Group K-without treatment, group K+ was given L-NAME, group P1 was given L-NAME + omega-3, group P2 was given L-NAME + vitamin E, and group P3 was given L-NAME + omega-3 + vitamin E. The L-NAME dose was 50 mg/kg/day. At the 19th day of gestation, the pregnancy was terminated and the blood serum was used for examination of ROS and placental tissue was collected for examination of VEGF using ELISA kit. The data were analyzed by one-way ANOVA and post hoc test Bonferoni. RESULTS: The analysis results found that there were significant differences between omega-3 and vitamin E supplements on ROS levels and placental VEGF levels. Administration of omega-3 only or combination with vitamin E can effectively improve endothelial function by increasing the level of VEGF. CONCLUSION: Omega-3 (DHA 120 mg, EPA 180 mg) and vitamin E (α-tocopherol 300 iu) supplement decreased ROS and increased placental VEGF on pre-eclampsia rats model.