Abstract
The dinucleoside analogues 24, 25, 28–30, and 33 associate in CDCl3 solution. Association constants, as determined from the concentration-dependent chemical shift for HN(3) of the uridine moiety and from thermodynamic parameters, range from 265 M−1 (33) to 3220 M−1 (30). The association of 31 in CDCl3 is too strong to be determined (concentration independent δ(HN(3)) of ca. 12.8 ppm) and the fully deprotected dimer 32 proved insufficiently soluble in CDCl3. This observation strongly evidences that structural differentiation of oligonucleotides and their analogues into backbone and nucleobases is not required for pairing. The dinucleotide analogues were prepared by O-alkylation of C(8)-unsubstituted or of C(8)-oxymethylated, partially protected adenosines by the C(6)-mesyloxy- or C(6)-halomethylated uridines 20–22, followed by partial or total deprotection.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.