Abstract
It is important to inhibit oxidative stress to maintain sperm motility during cryopreservation. The present study was performed to investigate the effects of supplementing oligomeric proanthocyanidins (OPC) and bamboo leaf flavonoids (BLF) or their combination as an extender for Simmental bull semen freezing. OPC, BLF, or their combination were added to the frozen diluent of bovine semen. Afterwards, computer-assisted semen analysis (CASA), detection of membrane functionality, acrosome integrity, mitochondrial integrity, CAT, SOD, GSH-PX, MDA, and ROS were conducted. The results showed that adding 50 mg/L OPC or 4 mg/L BLF could improve the quality of frozen sperm. Compared with 50 mg/L OPC alone, the combination of 50mg/L OPC and 2 mg/L BLF significantly increased the kinematic parameters of sperm, and sperm CAT, GSH-PX and SOD levels (p < 0.05), whereas the MDA of sperm was decreased (p < 0.05). These results indicated that compared to the addition of 50 mg/L OPC alone, a combination of 50 mg/L OPC and 2 mg/L BLF could further improve the quality of frozen semen. The results could provide theoretical data support for the development of a new protective agent and are significant for the cryopreservation of bovine semen in the future.
Highlights
Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China
In order to improve the quality of cryopreservation semen and reduce the effect of oxidative stress, adding antioxidants to cryopreservation diluents has become an important method [27]
Our results showed that adding 50 mg/L oligomeric proanthocyanidins (OPC) or 4 mg/L bamboo leaf flavonoids (BLF) could improve the quality of frozen semen
Summary
Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun 130118, China. OPC, BLF, or their combination were added to the frozen diluent of bovine semen. The results showed that adding 50 mg/L OPC or 4 mg/L BLF could improve the quality of frozen sperm. BLF significantly increased the kinematic parameters of sperm, and sperm CAT, GSH-PX and SOD levels (p < 0.05), whereas the MDA of sperm was decreased (p < 0.05). These results indicated that compared to the addition of 50 mg/L OPC alone, a combination of 50 mg/L OPC and 2 mg/L BLF could further improve the quality of frozen semen. In order to alleviate the oxidative stress caused by this imbalance, adding antioxidants to the diluent has become a necessary means [4]
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