Abstract
BackgroundDespite recent advances in colorectal cancer (CRC) diagnosis and population screening programs, the identification of patients with preneoplastic lesions or with early CRC stages remains challenging and is important for reducing CRC incidence and increasing patient’s survival.MethodsWe analysed 76 colorectal tissue samples originated from early CRC stages, normal or inflamed mucosa by label-free proteomics. The characterisation of three selected biomarker candidates was performed by immunohistochemistry on an independent set of precancerous and cancerous lesions harbouring increasing CRC stages.ResultsOut of 5258 proteins identified, we obtained 561 proteins with a significant differential distribution among groups of patients and controls. KNG1, OLFM4 and Sec24C distributions were validated in tissues and showed different expression levels especially in the two early CRC stages compared to normal and preneoplastic tissues.ConclusionWe highlighted three proteins that require further investigations to better characterise their role in early CRC carcinogenesis and their potential as early CRC markers.
Highlights
Despite recent advances in colorectal cancer (CRC) diagnosis and population screening programs, the identification of patients with preneoplastic lesions or with early CRC stages remains challenging and is important for reducing CRC incidence and increasing patient’s survival
The control group was composed of patients with diverticular diseases and the selected tissues treated were isolated within the diverticulitis zone itself [named diverticulitis inflammatory (DI)] or in the adjacent normal tissue [named diverticulitis healthy (DH)]
Proteomic discovery on early CRC stages Raw data and technical results In order to detect proteins differentially distributed through early CRC stages and controls, we generated protein extracts from the 76 Formalinfixed paraffin-embedded (FFPE) tissues regrouped
Summary
Despite recent advances in colorectal cancer (CRC) diagnosis and population screening programs, the identification of patients with preneoplastic lesions or with early CRC stages remains challenging and is important for reducing CRC incidence and increasing patient’s survival. Population screening programs for identification of patients with preneoplastic lesions or early CRC stages is important for reducing CRC incidence and for increasing patient’s survival. Dysplastic and neoplastic tissues regulate protein expression and produce protein profiles that might be correlated to precancerous or cancerous specific progression. To capture these events, it is adequate to investigate protein changes directly in the colon mucosa. Formalinfixed paraffin-embedded (FFPE) tissues stored in hospital biobanks represent a valuable resource for retrospective analysis as larger populations can be studied, enhancing the probability to identify significant and specific potential CRC biomarkers
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