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Abstract
BackgroundThe olfactomedin-like domain (OLFML) is present in at least four families of proteins, including OLFML2A and OLFML2B, which are expressed in adult rat retina cells. However, no expression of their orthologous has ever been reported in human and baboon.ObjectiveThe aim of this study was to investigate the expression of OLFML2A and OLFML2B in ocular tissues of baboons (Papio hamadryas) and humans, as a key to elucidate OLFML function in eye physiology.MethodsOLFML2A and OLFML2B cDNA detection in ocular tissues of these species was performed by RT-PCR. The amplicons were cloned and sequenced, phylogenetically analyzed and their proteins products were confirmed by immunofluorescence assays.ResultsOLFML2A and OLFML2B transcripts were found in human cornea, lens and retina and in baboon cornea, lens, iris and retina. The baboon OLFML2A and OLFML2B ORF sequences have 96% similarity with their human’s orthologous. OLFML2A and OLFML2B evolution fits the hypothesis of purifying selection. Phylogenetic analysis shows clear orthology in OLFML2A genes, while OLFML2B orthology is not clear.ConclusionsExpression of OLFML2A and OLFML2B in human and baboon ocular tissues, including their high similarity, make the baboon a powerful model to deduce the physiological and/or metabolic function of these proteins in the eye.
Highlights
The olfactomedin-like domain (OLFML) is present in at least four families of proteins, including olfactomedin like 2A (OLFML2A) and olfactomedin like 2B (OLFML2B), which are expressed in adult rat retina cells
The human OLFML2A and OLFML2B genes are located on chromosomes 9q33.3 and 1q23.3, respectively
RT‐PCR, molecular cloning and sequence analysis The OLFML2A and OLFML2B coding sequences were as expected (1947 and 2262 bp, respectively) and no other bands were detected as possible isoforms
Summary
The olfactomedin-like domain (OLFML) is present in at least four families of proteins, including OLFML2A and OLFML2B, which are expressed in adult rat retina cells. The olfactomedin (OLFM) family is a group of glycoproteins originally identified in bullfrogs (Rana catesbeiana) as the major component of the olfactory mucus layer, which surrounds the chemosensory dendrites of olfactory neurons [1]. Olfactomedinlikes (OLFML) are other members of the OLF family Some of their members are the glycoproteins OLFML2A and OLFML2B, known as photomedin-1 and photomedin-2, respectively, which were described in mice at 2005 [3]. The human OLFML2A and OLFML2B genes are located on chromosomes 9q33.3 and 1q23.3, respectively. These two genes are composed of at least 8 exons, and have a length of 37.3 and 40.7 kb, respectively [9]
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