Oleic acid Stimulates the Formation of Adipocyte-Like Cells from Bovine Satellite Cells via &amp;lt;i&amp;gt;G-Protein Coupled Receptor 43&amp;lt;/i&amp;gt; and &amp;lt;i&amp;gt;CCAAT/Enhancer-Binding Protein Beta&amp;lt;/i&amp;gt;

J Kim,K Chung,B J Johnson,L K Fuerniss

doi:10.4236/ojas.2020.104042

Abstract

Numerous physiological and pathological processes are controlled by free fatty acids, which act as signaling molecules in mammals. We hypothesized that oleic acid (Ole) might stimulate the formation of satellite-cell-derived intramuscular adipose tissue. The role of Ole as a ligand of G-protein-coupled receptor 43 (GPR43) was previously identified. Thus, the objective of the current study was to determine the effect of Ole on GPR43 and factors related to the adipogenic differentiation of bovine satellite cells (BSC). Treatments of 100 μM and 500 μM Ole tended to induce greater (P CCAAT/enhancer-binding protein β (C/EBPβ) compared to all other doses. The mRNA abundance of peroxisome proliferator-activated receptor γ (PPARγ) was not altered (P > 0.10) by treatment. The addition of 100 μM and 500 μM of Ole upregulated (P GPR43 mRNA expression. Protein level of GPR43 was increased (P C/EBPβ in response to Ole might suppress myogenic differentiation. After the treatment of cells with Ole, increased expression of GPR43 could lead to phosphorylation of 5’ AMP-activated protein kinase α (AMPKα). Altogether, the results indicated that increased Ole might stimulate adipose tissue accumulation within the skeletal muscle of cattle by promoting adipogenic differentiation and activation of GPR43 in satellite cells.

Highlights

The biology of intramuscular (IM) adipose tissue is widely discussed in meat-producing animals due to its economic value [1] [2] [3] [4]
The results suggested that satellite cells treated with oleic acid (Ole) were committed to exhibiting adipogenic characteristics
This result is in agreement with Asakura, Rudnicki and Komaki [12], who demonstrated that myoblasts were able to transdifferentiate into adipocytes with differentiation media containing myogenic and adipogenic inducers

Summary

Introduction

The biology of intramuscular (IM) adipose tissue is widely discussed in meat-producing animals due to its economic value [1] [2] [3] [4]. Progenitor cells of IM fat, muscle fibers, satellite cells, and fibroblasts are derived from a shared pool of mesenchymal stem cells [6]. These mononucleated myogenic cells play a pivotal role in postnatal muscle hypertrophy, and muscle regeneration by mesenchymal stem cell differentiation has been demonstrated in various animal models [7]. Joe, Yi, Natarajan, Le Grand, So, Wang, Rudnicki and Rossi [13] suggested that the subpopulation of the mononuclear mesenchymal cells called fibro/adipogenic progenitors within myogenic progenitors is possibly the primary source of fibroblasts and adipocytes in adult tissue

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Conclusion