Abstract
The locus and specific acitivity of oleate desaturase (ODS) activity of developing sunflower seeds has been studied using differential centrifugation. The ratio of activity recovered in the crude pellet, oil body and microsomal fractions was consistently about 3:1:2 throughout seed development. Microsomes always contained the highest specific activity of the enzyme, reaching a maximum at 20–25 days after flowering and declining rapidly thereafter. The change in desaturase specific activity (in vitro) with seed development slightly preceded linoleate accumulation (in vivo) but was synchronous with the changing levels of cytochrome b. Although the associated metabolic enzymes CDP-choline:1,2-diacylglycerol cholinephosphotransferase (CPT) and acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) reached maximum activities at the same time as the desaturase enzyme, these activities decreased less markedly during subsequent seed maturation. An unrelated microsomal enzyme, NADPH-cytochrome c-reductase (NCR), did not reach maximal activities until 40 days after flowering.
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