Oleanolic acid 및 그 유도체가 MC3T3-E1 조골세포주의 분화에 미치는 효과

Abstract

*Department of Food and Nutrition, Hanyang University, Seoul 133-791, Korea. **Department of Pharmacology, Hanyang University, Seoul 133-791, Korea.***School of Biomedical Science & Engineering, Hanyang University, Seoul 133-791, Korea. ABSTRACT : Ursolic acid, triterpenoid compound has been shown to stimulate osteoblast differentiation and enhance boneformation. In the present study, we examined the effects of similar triterpenoid compounds, oleanolic acid (OA) and itsderivatives, such as oleanolic acid acetate (OAA) and oleanolic acetate methyl ester (OAM) on the bone formation inMC3T3-E1 osteoblast cells. We determined cellular proliferation, alkaline phosphatase (ALP) activity, mineralization, andexpression of osteoblast specific genes and mitogen activated protein kinase phosphorylation. Treatment of 0.1-10µM OA,OAA, and OAM increased cellular proliferation, but not significantly increased as compared with dimethyl sulfoxide(DMSO). OA, OAA, and OAM at 5uM concentration enhanced ALP expression, mineralization, and osteocalcin (OCN)mRNA level. In conclusion, OA and its derivatives stimulated the osteoblast differentiation by increasing ALP, mineraliza-tion, and OCN mRNA expression. However, there were no significantly difference on osteoblast differentiation among treat-ment of OA, OAA, and OAM. Key Words : Oleanolic Acid, Oleanolic Acid Derivatives, Osteoblast Specific Ggene, Differentiation, Proliferation, MC3T3-E1 Cell