Oleanolic acid by MAPK/ERK signal transduction pathway in the proliferation and apoptosis of U87 MG cell in vitro

Abstract

Objective To investigate the role of oleanolic acid on the proliferation and Suppression of the migration of Malignant Glioma through the Deactivating MAPK/ERK Signaling Pathway in human Glioblastoma cell line U87.Methods The human Glioblastoma cells of U87 were treated with 25μg/ml,50μg/ml,80μg/rml,100μg/ml,150μg/ml,and 200μg/ml of OA for 24h、48h、and 72h.Cell viability of the proliferation was assessed with MTＴ assay.The migration capabilities were observed with the scratch test.Flow cytometry was adopted to analyze cell cycle and apoptosis.Western blotting was used to detect the activation of the MAPK/ERK Signaling Pathway.Results The morphology of U87 MG cells changed with extensive cell detachment,after 48h treatment of 50μg/ml and a higher concentration of OA.Its growth inhibitory rates on U87 MG cells were up to 100％ under 72h treatment on 150μg/ml and 200μg/ml,and compared with control group P ＜0.01.The48h treatment of 25μg/ml OA reduced the amount of cells migrating into the wounding area.The percentage of cells undergoing G0/G1phase rose from 64.23％ to 75.03％ after the 48h treatment of 50μg/ml OA,indicating that G0/G1anest took place.At the same concentration,the apoptotic rate of U87 MG cells was 18.77％.The phosphorylations of MEK and ERK were both suppressed in U87 MG cells.Conclusion OA can significantly inhibit the growth of U87 cell proliferation,a certain quantity effect characteristic; it can obviously inhibit U87 cell migration,and inhibit the MAPK/ERK signaling pathways leading to the activation.Oleanolic acid has the effects of inhibition on U87 cell proliferation and migration capabilities,also it can induct cell apoptosis through deactivating the MAPK/ERK signaling pathway. Key words: Glioblastoma; Oleanolic acid; MAPK/ERK signaling pathway ; Apoptosis ; U87 MG cell