Abstract
Ethnopharmacological relevanceCoreopsis tinctoria Nutt has various medical and functional properties and its flower is widely used as health-care tea to decrease blood glucose and to lower blood lipids. However, the quorum sensing (QS) inhibition activity of Coreopsis tinctoria Nutt flower remains unclear.Aim of the study: To assess inhibitory activity against quorum sensing by Chromobacterium violaceum, to identify the chemical composition of the extracts and to disclose the action mechanism of separated compound. Material and methodsViolacein inhibition assays were performed in 96-wells microplates. The compounds extracted from Coreopsis tinctoria Nutt flower were separated and purified by various chromatography techniques. Respectively, thin layer chromatography (TLC, GF254), mass spectrometer (Agilent 1100 Series LC/MSD Trap SL), Medium-pressure automatic purification system (Buscisepacore C 620, Switzerland), High performance liquid chromatography (HPLC, Shimadzu LC-20AD, Japan), Liquid preparation Chromatography (Waters2545, USA). The chemical structures were identified by nuclear magnetic resonance (NMR, Bruker AV-500, Germany) technique. The inhibitory mechanism of okanin against C. violaceum quorum sensing was detected by quantitative real-time PCR (qRT-PCR). ResultsQuorum sensing regulates production of bacterial virulence factors, thereby making it an intriguing target for attenuating bacterial pathogenicity. In this study, anti-QS activity of Coreopsis tinctoria Nutt methanol fraction (CTM) was investigated against C. violaceum ATCC12472. CTM showed an inhibitory effect on the QS-mediated virulence factors production such as violacein in C. violaceum without effect on growth rate. Also, okanin was isolated from CTM and its potential of anti-QS was confirmed after observing a significant reduction of violacein production in C. violaceum. An attempt was made to assess the effect of okanin on vioABCDE expression in C. violaceum to disclose acting mechanisms. ConclusionsThe results of this study contribute to validate an inhibitory effect of Coreopsis tinctoria Nutt flower on quorum sensing by Chromobacterium violaceum and to determine the compound responsible for inhibition. Also, the inhibitory effect was achieved in tandem with the down-regulation of vio operon.
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