OIP5-AS1 accelerates apoptosis of hippocampal neurons in cell models of epilepsy by modulating MiR-128-3p/BAX axis

Abstract

As a chronic neurological disorder, epilepsy (EP) is characterized with recurrent and unexplained epileptic seizures. Mounting evidence demonstrated that long non-coding RNAs (lncRNAs) are associated with EP. This paper intended to study the role and mechanisms of OIP5 antisense RNA 1 (OIP5-AS1) in EP.Quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze relative RNA level. Cell viability was unclosed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) experiment. The activity of caspase-3/9 was investigated to measure cell apoptosis. Subcellular fractionation assay was carried out to uncover the subcellular location. RNA pulldown, luciferase reporter and RNA-binding protein immunoprecipitation (RIP) assays were applied to disclose the underlying mechanisms of OIP5-AS1.Result shows OIP5-AS1 is overexpressed in EP cell models and mainly located in cytoplasm. OIP5-AS1 knockdown impairs cell apoptosis in EP cell models. OIP5-AS1 regulates cell apoptosis in EP cell models by binding to microRNA-128-3p (miR-128-3p). OIP5-AS1 interacts with miR-128-3p to overexpress BCL2-Associated X (BAX), thereby modulating cell apoptosis in EP cell models.OIP5-AS1 accelerates apoptosis of hippocampal neurons in cell models of EP by modulating miR-128-3p/BAX axis. Investigating OIP5-AS1/miR-128-3p/BAX regulatory axis can contribute to deepening the understanding of EP.