Oil-degrading properties of a psychrotolerant bacterial strain, Rhodococcus sp. Y2-2, in liquid and soil media.

Abstract

The aim of this study was to investigate oil-degrading ability of newly isolated strain Rhodococcus Y2-2at low temperature. Rhodococcus sp. Y2-2 was isolated from oil-contaminated soil sampled at the end of winter using a newly developed transwell plate method. In the liquid phase, the oil-degradation efficiency of strain Rhodococcus sp. Y2-2 was about 84% with an initial concentration of 1500ppm TPH (500ppm each of kerosene, gasoline, and diesel) when incubated for 2 weeks under optimal conditions: 10°C, pH 7, and 0.5g L- 1 inoculum. In the soil phase, the isolate showed 80% oil degradation efficiency using glucose as a carbon source, with an initial concentration of 4000ppm TPH and the addition of water during 14 days of incubation at 10°C. Additionally, the degradation efficiency of the isolate was increased by the addition of mixture of surfactant alpha olefin sulfonate and gelatin, although strain Y2-2 also produced many biosurfactant components. This study shows Rhodococcus sp. Y2-2 can degrade oil components both in liquid and soil media by consuming kerosene, gasoline, and diesel as a carbon and energy source. Therefore, the crude oil-degrading ability of Rhodococcus sp. Y2-2at low temperature provides proper bioremediation tool to clean up oil-contaminated sites especially in cold area or during winter season.