O-GlcNAc informatics: advances and trends.

Abstract

As a post-translational modification, protein glycosylation is critical in health and disease. O-Linked β-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation), as an intracellular monosaccharide modification on proteins, was discovered 40 years ago. Thanks to technological advances, the physiological and pathological significance of O-GlcNAcylation has been gradually revealed and widely appreciated, especially in recent years.O-GlcNAc informatics has been quickly evolving. Clearly, O-GlcNAc informatics tools have not only facilitatedO-GlcNAc functional studies, but also provided us a unique perspective on proteinO-GlcNAcylation. In this article, we review O-GlcNAc-focused software tools and servers that have been developed forO-GlcNAc research over the past four decades. Specifically, we will (1) survey bioinformatics tools that have facilitated O-GlcNAc proteomics data analysis, (2) introduce databases/servers forO-GlcNAc proteins/sites that have been experimentally identified by individual research labs, (3) describe software tools that have been developed to predictO-GlcNAc sites, and (4) introduce platforms cataloging proteins that interact with the O-GlcNAc cycling enzymes (i.e., O-GlcNAc transferaseand O-GlcNAcase). We hope these resources will provide useful information to both experienced researchers and new incomers to the O-GlcNAc field. We anticipate that this review provides a framework to stimulate the future development of more sophisticated informatic tools for O-GlcNAc research.