Abstract
In a recent communication (Smith & Blackham, 1957) efforts to determine the optimum conditions for the hydrolytic cleavage of oestrogen conjugates as they occur in the urine of women were reported. Twelve late pregnancy urines from 3 normally pregnant women were divided into aliquots and the results of six different hydrolytic measures compared. Evaluation of the results was acquired by chemical assays for oestradiol, oestrone and oestriol by the Brown (1955) procedure. Maximum recovery of all three was obtained by glucuronidase followed by mild acid hydrolysis. Glucuronidase alone recovered 80 to 88 % of the maximum and was the only single step measure that gave a satisfactory degree of uniformity in the »percentage of the maximum« recovered. It is the object of the present communication to report bio-assays on this same material. Preliminary bio-assays findings on some of the urines revealed the presence of considerably more oestrogenic potency than could be
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