Abstract
In recent years, the use of botanical agents to prevent skin damage from solar ultraviolet (UV) irradiation has received considerable attention. Oenanthe javanica is known to exert anti-inflammatory and antioxidant activities. This study investigated photoprotective properties of an Oenanthe javanica extract (OJE) against UVB-induced skin damage in ICR mice. The extent of skin damage was evaluated in three groups: control mice with no UVB, UVB-exposed mice treated with vehicle (saline), and UVB-exposed mice treated with 1% extract. Photoprotective properties were assessed in the dorsal skin using hematoxylin and eosin staining, Masson trichrome staining, immunohistochemical staining, quantitative real-time polymerase chain reaction, and western blotting to analyze the epidermal thickness, collagen expression, and mRNA and protein levels of type I collagen, type III collagen, and interstitial collagenases, including matrix metalloproteinase (MMP)-1 and MMP-3. In addition, tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-2 protein levels were also assessed. In the UVB-exposed mice treated with extract, UV-induced epidermal damage was significantly ameliorated. In this group, productions of collagen types I and III were increased, and expressions of MMP-1 and MMP-3 were decreased. In addition, TNF-α and COX-2 expressions were reduced. Based on these findings, we conclude that OJE displays photoprotective effects against UVB-induced collagen disruption and inflammation and suggest that Oenanthe javanica can be used as a natural product for the treatment of photodamaged skin.
Highlights
Skin aging is divided into intrinsic aging and photoaging caused by repeated exposure to ultraviolet (UV) radiation
The effect of Oenanthe javanica extract (OJE) on the UVB-exposed dorsal skin was assessed by analyzing levels of erythema/hemorrhage, scarring/dryness, edema, and excoriation/erosion (Figure 1)
Skin is the largest organ in the human body and acts as a primary physical barrier to the enviroSnkminenist. thTehluasr,geskstinorisgaenxpinostehde thouUmVanligbhotdythraonudghactssolaasr arapdriaimtioanry. pThhyrseiecaml abianrrsiuerbttyoptehse oefnUvVirroandmiaetniotn
Summary
Skin aging is divided into intrinsic aging and photoaging caused by repeated exposure to ultraviolet (UV) radiation. Intrinsic aging is characterized by smooth, pale, and finely wrinkled skin, and photoaging includes coarse deep wrinkles, dyspigmentation, and telangiectasia [1]. Alterations in collagen fibers of the dermis are thought to cause the wrinkles observed in intrinsic aging and in photoaging due to an imbalance between collagen synthesis and degradation. It is thought that UV-induced expressions of MMPs are related to the deep wrinkles seen in photoaged skin because UV light induces expressions of MMPs in the skin [2]. UV radiation induces expressions of MMP-1, MMP-3, and MMP-9 in the skin, and it has been suggested that MMP-1 initially disrupts collagen types I and III, after which MMP-3 and/or MMP-9 further degrade the disrupted collagen proteins [3]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
