Abstract
Levels of cytochrome P-450 and related enzyme activities in Octopus pallidus were investigated for the first time to assess their potential for use as biomarkers of xenobiotic exposure. Spectral analysis revealed cytochrome P-450 in the digestive gland microsomes. Enzyme assays 7-ethoxyresorufin O-deethylase and 7-ethoxycoumarin O-deethylase were optimised with respect to temperature, pH and incubation time and NADPH cytochrome c reductase assays were performed. Corn oil was demonstrated to have an inhibitory effect on octopus cytochrome P-450 system. The study with Aroclor 1254 suspended in glycerol (100 mg/kg) revealed an elevation of 7-ethoxycoumarin O-deethylase activity, which would indicate cytochrome P-450 was induced.
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