Abstract
An abnormal isoform of prion protein (PrP Sc), which is composed of the same amino acids as cellular PrP (PrP C) and has proteinase K (PK)-resistance, hypothetically converts PrP C into PrP Sc. To investigate the region important for PrP Sc production, we examined the levels of PrP Sc in PrP gene-deficient cells (HpL3-4) expressing PrP C deleted of various regions including the octapeptide repeat region (OR) or hydrophobic region (HR). After Chandler or Obihiro prion infection, PrP Sc was produced in HpL3-4 cells expressing wild-type PrP C or PrP C deleted of HR at an early stage and further reduced to below the detectable level, whereas cells expressing PrP C deleted of OR showed no PrP Sc production. The results suggest that OR of PrP C is required for the early step of efficient PrP Sc production.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.