Oct4B, CD90, and CD73 are upregulated in bladder tissue following electro-resection of the bladder

Takumi Takeuchi,Yukihiro Okuno ,Akiko Tonooka,Mami Hattori-Kato,Koji Mikami

doi:10.46582/jsrm.1201003

Abstract

We tested the hypothesis that stimulation by electro-resection of bladder tissue induces stem cells in the tissue repair process. After primary transurethral resection of a bladder tumor and surrounding tissue (TUR-Bt), second TUR-Bt was performed. Tissues excised by second TUR-Bt were immunohistochemically stained for Oct4, a marker of pluripotency, and for CD90 and CD73, markers of mesenchymal stromal cells, when no bladder tumor cells remained. Oct4B protein was sporadically stained in the cytoplasm of interstitial cells in four out of eight cases. CD90 and CD73 are upregulated in interstitial and vascular endothelial cells without CD45 expression. Mesenchymal stromal cells, but not pluripotent stem cells, may be mainly involved in bladder tissue repair.

Highlights

In 2014, Obokata et al, reported a cellular reprogramming phenomenon with acid treatment called stimulus-triggered acquisition of pluripotency (STAP)
Eight bladder paraffin-embedded specimens of secondary resections without remaining bladder tumors on examination with hematoxylineosin staining were immunohistochemically stained using anti-human Oct4 rabbit monoclonal antibody raised against amino acids 250-350 of human origin (EPR2054, Abcam, Cambridge, UK), anti-human Oct4 mouse monoclonal antibody raised against amino acids 1-134 of human origin, anti-human CD90/Thy1 rabbit monoclonal antibody (EPR3132, Abcam, Cambridge, UK), anti-human CD73 mouse monoclonal antibody, and anti-human CD45 mouse monoclonal antibody (IR751, Dako Japan, Tokyo, Japan)
In four out of the eight cases, Oct4 protein was sporadically stained with EPR2054 in the cytoplasm of interstitial cells located in the specimens of second TUR-Bt (Figure 3 and Figure 4), but sc-5279 did not detect Oct4A protein in the specimens, revealing that Oct4 detected by EPR2054 is Oct4B

Summary

Introduction

In 2014, Obokata et al, reported a cellular reprogramming phenomenon with acid treatment called stimulus-triggered acquisition of pluripotency (STAP). The POU family transcription factor Oct (octamer-binding transcription factor 4) is an essential regulator of pluripotency and is of central significance in nuclear reprogramming[2]. It is one of the four reprogramming Yamanaka factors generating induced pluripotent stem cells[3]. Mesenchymal stromal cells (MSCs) are a rare population of non-hematopoietic stromal cells that reside in the bone marrow and connective tissues. They have the potential to differentiate into mesenchymal tissues such as bone, cartilage, muscle, and adipose tissues and, can be significant in tissue repair[4]. CD90 (thymus cell antigen 1) and CD73 (ecto-5'nucleotidase) are surrogate positive markers of MSCs, while MSCs lack CD45 (leukocyte common antigen) expression[5, 6]

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