Abstract
Phase-resolved OCT and fluorescence microscopy were used simultaneously to examine stereotypic patterns of neural activity in the isolated Drosophila central nervous system. Both imaging modalities were focused on individually identified bursicon neurons known to be involved in a fixed action pattern initiated by ecdysis-triggering hormone. We observed clear correspondence of OCT intensity, phase fluctuations, and activity-dependent calcium-induced fluorescence.
Highlights
Current methods for detection of neural activity include functional magnetic resonance imaging [1, 2], intrinsic optical imaging [3, 4], near infrared spectroscopy [5, 6], calcium- [7,8,9] and voltage-sensitive dyes [10, 11], and a wide variety of electrodes [12,13,14]
In vitro neuronal imaging was performed on the isolated central nervous system (CNS) of pre-pupae
As there was significant lateral scanning involved with volumetric acquisition, only changes in optical coherence tomography (OCT) intensity were analyzed in this set of experiments
Summary
Current methods for detection of neural activity include functional magnetic resonance imaging [1, 2], intrinsic optical imaging [3, 4], near infrared spectroscopy [5, 6], calcium- [7,8,9] and voltage-sensitive dyes [10, 11], and a wide variety of electrodes [12,13,14]. G. Fujimoto, “In vivo functional imaging of intrinsic scattering changes in the human retina with high-speed ultrahigh resolution OCT,” Opt. Express 17(5), 3861–3877 (2009). “In vivo imaging of intrinsic optical signals in chicken retina with functional optical coherence tomography,” Opt. Lett. A. Boas, “Depth-resolved imaging of functional activation in the rat cerebral cortex using optical coherence tomography,” Opt. Lett.
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