Ochrobactrum teleogrylli sp. nov., a pesticide-degrading bacterium isolated from the insect Teleogryllus occipitalis living in deserted cropland.

Abstract

A Gram-stain-negative, non-spore-forming, motile, aerobic, rod-shaped bacteria strain, designated LCB8T, was isolated from the insect Teleogryllus occipitalis captured from a deserted cropland in Shuangliu district, Chengdu, PR China. Phylogenetic analysis on the basis of 16S rRNA gene sequence indicated that the strain represented a member of the genus Ochrobactrum, family Brucellaceae, class Alphaproteobacteria. Ochrobactrum pecoris CCUG 60088T (97.9 %) and Ochrobactrum haematophilum CCUG 38531T (98.8 %) were identified as the most closely related phylogenetic neighbours of strain LCB8T. The novel strain was able to grow at salt concentrations of 0-4.5 % (w/v), pH 5-9 and temperatures of 20-42 °C. The major quinone system was ubiquinone Q-10, the major fatty acids were C18 : 1ω7c, C16 : 0 and C18 : 0. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and four undefined aminolipids. The major polyamines were putrescine and spermidine. Genome sequencing revealed a genome size of 4.76 Mbp and a DNA G+C content of 57.1 mol%. These phenotypic, genotypic and chemotaxonomic traits excellently supported the affiliation of LCB8T to the genus Ochrobactrum. Pairwise determined whole-genome average nucleotide identity (ANI) values indicated that strain LCB8T represents a novel species, for which we propose the name Ochrobactrum teleogrylli sp. nov. with the type strain LCB8T (=KCTC 72031T=CGMCC 1.13984T).