Abstract
Ochratoxin A (OTA), a mycotoxin found in many foods worldwide, causes nephrotoxicity, hepatotoxicity, and immunotoxicity, both in vitro and in vivo. In the present study, we explored the cytotoxic effects exerted by OTA on the blastocyst stage of mouse embryos, on subsequent embryonic attachment, on outgrowth in vitro, and following in vivo implantation via embryo transfer. Mouse blastocysts were incubated with or without OTA (1, 5, or 10 μM) for 24 h. Cell proliferation and growth were investigated using dual differential staining; apoptosis was measured using the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay; and embryo implantation and post-implantation development were assessed by examination of in vitro growth and the outcome of in vivo embryo transfer, respectively. Blastocysts treated with 10 μM OTA displayed a significantly increased level of apoptosis and a reduction in total cell number. Interestingly, we observed no marked difference in implantation success rate between OTA-pretreated and control blastocysts either during in vitro embryonic development (following implantation in a fibronectin-coated culture dish) or after in vivo embryo transfer. However, in vitro treatment with 10 μM OTA was associated with increased resorption of post-implantation embryos by the mouse uterus, and decreased fetal weight upon embryo transfer. Our results collectively indicate that in vitro exposure to OTA triggers apoptosis and retards early post-implantation development after transfer of embryos to host mice. In addition, OTA induces apoptosis-mediated injury of mouse blastocysts, via reactive oxygen species (ROS) generation, and promotes mitochondrion-dependent apoptotic signaling processes that impair subsequent embryonic development.
Highlights
Ochratoxin A (OTA) is a mycotoxin produced principally by ubiquitous strains of Aspergillus and Penicillium [1]
We found that OTA suppressed embryonic cell proliferation during the blastocyst stage predominantly by inducing apoptosis of the inner cell mass (ICM)
OTA at 10 μM clearly induced apoptosis (Figure 1A); the level of cell death was nine-fold higher in OTA-treated blastocysts than in untreated controls (Figure 1B)
Summary
Ochratoxin A (OTA) is a mycotoxin produced principally by ubiquitous strains of Aspergillus and Penicillium [1]. OTA is one of the most common food-contaminating mycotoxins, and is often isolated from beans, grains, cereals and spices. OTA has contaminated coffee, grape juice, wine, beer and bread [2]. It is very difficult to completely avoid dietary exposure to OTA because the chemical occurs widely in various food stuffs [3,4]. It is important to study the adverse effects of OTA on humans. Previous studies have found that OTA is nephrotoxic and hepatotoxic, and causes neurodegenerative disease [5]. Recent work has shown that OTA creates oxidative stress in several regions of the mouse midbrain and hippocampus, compromising brain development [6]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
