Ocena wpływu kwasu α-liponowego na obraz morfologiczny serca i nerki królików karmionych paszą z dodatkiem utlenionego oleju rzepakowego

Abstract

Introduction. The peroxidation of lipids is the effect of subjecting them to the high temperature. The studies have shown that the created oxidative stress can be counteracted by strengthening the body’s antioxidative system by introducing antioxidant vitamins or non-vitamin compounds into the body. One of the best antioxidants that works in both the water and lipid phase is an α-lipoic acid. Aim. The aim of the study was to assess the influence α-lipoic acid on morphological changes in heart and kidneys of rabbits exposed to oxidized or non-oxidized rapeseed oil. Material and methods. The research was carried out on 36 rabbits of the Szynszyl breed. The animals were divided into 6 groups of 6 individuals. They were given the standard diet Labofeed Kamet (DS). They were drinking water at libitum. The animals in the control group received standard Labofeed Kamet feed, in group I (DS + LA) a-lipoic acid (LA) was added to the feed at a dose of 10 mg/kg, in group II (DS + NOR) ? there was added 10% non-oxidized rapeseed oil (NOR), in group III (DS + UOR) ? 10% oxidized rapeseed oil (UOR) was given, in group IV (DS + NOR + LA) the rabbits received feed enriched with non-oxidized rapeseed oil and α-lipoic acid, and group V (DS + UOR + LA) was enriched with oxidized rapeseed oil and α-lipoic acid in the same amounts as in the each group. After the experiment was completed and the animals were euthanised, the kidneys and hearts of the rabbits were collected for histopathological evaluation. Results. A high-fat diet causes adverse changes in both examined organs. The administration of α-lipoic acid showed a preventive effect on changes observed in organs. It was fully effective in the case of exposure of rabbits to non-oxidized rapeseed oil. When exposed to an oxidized oil, persistence of changes of lesser intensity were observed than when exposed only to oxidized oil. Conclusions. Dietary supplementation with antioxidants should be considered when exposed to oxidized lipids.