Abstract
In the present study 200 milk samples (50 each from cow, buffalo, market and mastitic milk) were collected from Hyderabad. Among the isolated presumptiveListeria spp., all the isolates were identified asListeria monocytogenes and showed a prevalence of 6, 8, 10 and 12%, respectively. Through serotyping analysis all isolates were confirmed asListeria genus, since they agglutinated with polyvalent ‘O’ serum; 12 of the 18L. monocytogenes isolates were ascribed to serotype 1, and 6 to serotype 4. Furthermore, the pathogenicity was investigated in rabbit and mice through Anton and inoculation tests, respectively. The organism produces keratoconjunctivitis in rabbit while it causes death and presence of necrotic foci in liver, spleen and kidneys of mice.Listeria monocytogenes was found sensitive to ampicillin, chloramphenicol, ciprofloxacin, gentamycin and kanamycin.
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