Occurrence of VIM-4 metallo-β-lactamase-producing Pseudomonas aeruginosa in an Algerian hospital.

Manel Merradi,Taha Menasria,Didier Hocquet,Nouzha Heleili,Pascal Cholley,Ahmed Kassah-Laouar,Marlène Sauget,Ammar Ayachi

doi:10.3855/jidc.10679

Abstract

Pseudomonas aeruginosa is one of the most common nosocomial pathogens, known with a wide resistance to antimicrobials. Carbapenemases producing Pseudomonas aeruginosa is a growing global public health concern as this pathogen is easily transmissible among patients. Metallo-Beta-lactamases is the most important class of these carbapenemases with their broad-spectrum resistance profile. This study was conducted to investigate the prevalence of MBL-producing P. aeruginosa collected in an Algerian hospital. All Metallo-β-lactamase (MBL)-producing P. aeruginosa isolates recovered from patients during a 2 years period (2015-2016) were studied using a combination of phenotypic and molecular typing methods (susceptibility testing, molecular characterization of carbapenemase-encoding genes, multi-locus sequence typing and pulsed-field gel electrophoresis). A total of twenty-six MBL producing P. aeruginosa of 188 isolates were investigated. The burns unit ranked in the first position of the majority of identified cases with 73.07%. About 73.07% of total MBL isolates were mainly isolated from pus samples. The studied isolates were subjected to the molecular typing, in which 4 different Dra1-PFGE patterns and 3 sequences type were assigned (ST244, ST381, and ST1076), and all isolates were revealed positive for VIM-4. We report the third description of blaVIM-4 in Algeria indicating the emergence and spread of carbapenemase-encoding genes among P. aeruginosa in the hospital environment.

Highlights

Pseudomonas aeruginosa is one of the most common nosocomial pathogens, known with a wide resistance to antimicrobials
Our results showed that P. aeruginosa strains were mainly isolated in burns unit which is due to the fact that skin damage is a factor contributing to P. aeruginosa infections [20]
Few reports of VIM class MBL isolates in Algeria were depicted, the first one was the bla VIM -19described in five Enterobacteriaceae clinical isolates [9], the blaVIM-2 has been noted in P.aeruginosa clinical strains [10,11] and the blaVIM-4 gene that has been recently reported among clinical isolates of P. aeruginosa [13, 34], by this study we report the third detection of VIM4 gene carrying P. aeruginosa in Algeria indicating its widespread in the hospital environment

Summary

Introduction

Pseudomonas aeruginosa is one of the most common nosocomial pathogens, known with a wide resistance to antimicrobials. Carbapenemases producing Pseudomonas aeruginosa is a growing global public health concern as this pathogen is transmissible among patients. Methodology: All Metallo-β-lactamase (MBL)-producing P. aeruginosa isolates recovered from patients during a 2 years period (2015-2016) were studied using a combination of phenotypic and molecular typing methods (susceptibility testing, molecular characterization of carbapenemase-encoding genes, multi-locus sequence typing and pulsed-field gel electrophoresis). Its high genetic plasticity enables it to colonize a wide variety of environments and infect almost all anatomical sites [2]. It is responsible for severe hospital-acquired infections especially for the patients compromised by underlying disease, age or immune deficiency (burns, cystic fibrosis, meningitis, abscess, urinary tract infections, catheter-associated infections, and ocular infections) [3]. As a result of the intensive use of these molecules, P. aeruginosa jeopardizes therapy and developed a resistance that threatened health care systems [5]

Methods

Discussion

Conclusion