Occurrence of two forms of Y chromosome in rainbow trout ( Oncorhynchus mykiss) males from Rutki strain

Abstract

Morphologically differentiated sex chromosomes are found in only few salmonid species. Some populations of rainbow trout, Oncorhynchus mykiss, exhibit chromosomal polymorphism related to sex. We found sex-related chromosomal polymorphism in fish from the synthetic Rutki strain, Poland, in approximately 85% ( n = 22) of examined males (XY-like) whereas the remaining males ( n = 4) possessed chromosomes similar to these observed in females (XX-like). To investigate whether males possessing XX-like chromosomes were genotypic males or genotypic females with altered phenotypic sex, androgenetic progeny of four males (representing both XY-like and XX-like forms, n = 2 + 2) was examined. Androgenetic progeny (F 1) of all four fathers consisted of both phenotypic females and males. F 1 male progeny of two fathers showed XX-like chromosomes whereas F 1 male progeny of the other two fathers possessed YY-like (supermale) chromosomes. F 1 were reared further until they were sexually mature. Two males from each of four F 1 families were used to produce F 2 androgenetic and control F 2 generation. All F 2 individuals, androgenetics and control, were phenotypic males at sexual maturation. The results indicate that males possessing XX-like chromosomes are genetic males and they are not sex-reversed females. Thus, the Y chromosome can exist in different morphological forms in farmed rainbow trout. The Y S chromosome (shorter form, unlike the X chromosome) has a shorter or absent p-arm and lacks the pericentromeric cluster of AT-rich chromatin and the 5S rDNA sequences that are found in the Y L chromosome (longer form, like the X chromosome).