Abstract
A turnip mosaic virus (TuMV) isolate from asymptomatic Phalaenopsis sp. was detected by indirect ELISA. Its presence was confirmed by RT-PCR with a pair of degenerate primers whose design was based on reported coat protein gene sequences. Further analysis of the genomic sequence of this isolate designated as TuMV-ZH1 (GenBank accession No. KF246570) showed a high nucleotide sequence identity with isolate Lu2 (96.7%) and CHN 12 (96.3%) from China. Phylogenetic analysis indicated that TuMV-ZH1 belongs to the world-B lineage. To the best of our knowledge, the above may be the first indication that TuMV infects orchids in China. In addition, proteins P1 and P3 of TuMV-ZH1 are highly mutated as previously reported for other TuMV isolates.
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