Abstract
The activity of thiosulphate sulphurtransferase (rhodanese, EC 2.8.1.1) in randomly isolated bacteria and mieromycetes of the humus horizon in a spruce forest was followed. Bacteria isolated on soil extract agar (70 cultures) did not yield unambiguous results due to poor growth of the cultures. Of 63 bacterial cultures on meat-peptone agar almost 80 % of the isolates produced the enzyme. Sixty-three % of isolates had a concentration higher than 5 nkat/L and 35 % higher than 20 nkat/L. The soil rhodanese activity can be explained by their presence. None of 30 isolates of micromycetes produced thiocyanate.
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