Occurrence of the mucinous differentiation antigen CA125 in genital tract and conductive airway epithelia of diverse mammalian species (rabbit, dog, monkey)

Abstract

Abstract CA125 is a human tumor-associated antigen of coelomic epithelial origin. In the present study, immunohistochemical analysis of normal rabbit, dog, and monkey tissues using monoclonal antibody OC125, revealed that in these animals positive staining for CA125 is found in all tissues that produce this mucin-like glycoprotein in man, i.e., the peritoneal and pleural mesothelium, the different Müllerian-duct-derived epithelia of the female genital tract, and the epithelium of trachea, bronchi, bronchioli, and mucoserous respiratory glands; CA125 was also detected in some ductal and acinar cells of the dog mammary gland. Without trypsin treatment of sections, staining was predominantly localized on the apical cell surface of all mentioned cell types. After treatment, mucih droplets inside respiratory mucous cells were also positively stained. In all cases, staining was associated with material positive for periodic acid-Schiff (PAS) and Alcian blue. In rats, its presence could not be demonstrated. Our results show that the CA125 epi-tope is not restricted to man and that its expression throughout different animal species is associated with well-defined tissue compartments. The expression of the mucous differentiation antigen CA125 in several common laboratory animals provides new opportunities for the experimental study of its biological significance.