Abstract
This study aimed to verify the occurrence of Shiga toxin-producing Escherichia coli (STEC) strains in three distinct anatomic parts of the stable fly Stomoxys calcitrans by multiplex polymerase chain reaction (PCR Multiplex). According to the results obtained, E. coli was identified in 19.5% of the stable flies. Shiga toxin genes were detected in 13% of the E. coli isolated, most frequently from the surface, followed by abdominal digestive tract and mouth apparatus of insects, respectively. This is the first study to detect presence of STEC in Stomoxys calcitrans in Brazil; it has also revealed the potential role of stable flies as carriers of pathogenic bacterial agents.
Highlights
Muscidae species such as the stable fly, Stomoxys calcitrans, stands out among the main insects that disperse infectious agents to farm animals
This study aimed to verify the occurrence of Shiga toxin-producing Escherichia coli (STEC) strains in three distinct anatomic parts of the stable fly Stomoxys calcitrans by multiplex polymerase chain reaction (PCR Multiplex)
This is the first study to detect presence of STEC in Stomoxys calcitrans in Brazil; it has revealed the potential role of stable flies as carriers of pathogenic bacterial agents
Summary
Muscidae species such as the stable fly, Stomoxys calcitrans, stands out among the main insects that disperse infectious agents to farm animals. Besides using cattle feces and secretions for food and reproduction during its life phases, this fly takes blood meals from various animals. It can spread a number of infectious agents such as parasitic forms of helminths, protozoa, fungi and bacteria. Parasitism by this fly has been associated with the outbreak of ailments among cattle (GRACZYK et al, 2001; BITTENCOURT; CASTRO, 2004). Cattle do not usually present any clinical symptoms Products from these animals, such as milk and meat, are potential sources of this agent, mainly when these products are eaten without proper heat treatment, www.cbpv.com.br/rbpv v. Products from these animals, such as milk and meat, are potential sources of this agent, mainly when these products are eaten without proper heat treatment, www.cbpv.com.br/rbpv v. 22, n. 2, abr.-jun. 2013
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