Abstract
Abstract Periodate-thiobarbituric acid reaction-positive substances were found in the strong acid hydrolysates of the lipopolysaccharide (LPS) from Bacteroides gingivalis 381. They were not identical to 2-keto-3-deoxyoctonate (KDO) in high-voltage paper electrophoresis (HVPE), their electrophoretic mobilities relative to KDO being 1.54 and 1.80, respectively. Alkaline phosphatase treatment and HVPE demonstrated that they are some kind of O-phosphorylated derivatives of KDO; in particular, the slow-moving component is identical, at least in HVPE, to 5-O-phosphoryl-KDO isolated from the strong acid hydrolysates of Bordetella pertussis (phase I) LPS.
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