Occurrence of nitrite-dependent anaerobic methane oxidation bacteria in the continental shelf sediments

Abstract

Nitrite-dependent anaerobic methane oxidation (N-damo) is a key bioprocess coupling global carbon and nitrogen cycles and is mediated by NC10 bacteria. So far, the distribution of N-damo bacteria in marine sediments has rarely been reported. In this study, the sediments from the Bohai Sea, Yellow Sea and East China Sea were taken as the research objects, and the ecological distribution of N-damo bacteria was investigated by quantitative PCR and amplicon sequencing. Quantitative PCR results demonstrated that the highest average copy number of N-damo bacterial 16S rRNA gene was in the Bohai Sea, followed by the East China Sea, while the lowest was observed in the Yellow Sea. Based on the OTU numbers, the N-damo bacterial diversity was highest in East China Sea, followed by the Bohai Sea, while lowest in the Yellow Sea. The N-damo bacterial community structure exhibited an obvious spatial distribution among the three seas. Sediment nitrite nitrogen content is the key environmental factor affecting the abundance and diversity of N-damo bacteria, and sediment ammonia nitrogen content is the key environmental factor affecting the community structure of N-damo bacteria.