Abstract
Abstract—A substance identical with N‐acetyl‐l‐alanine was isolated from an aqueous extract of human brain by a combination of paper and ion‐exchange Chromatography. The isolated substance did not react with ninhydrin reagent but yielded alanine upon acid hydrolysis. An acetyl hydrazide was identified by paper chromatography of hydrazinolysates of the isolated substance and N‐acetyl‐l‐alanine. The unknown alanine had the l‐configuration. The results of elementary analysis of the isolated compound were in full accord with the analysis calculated for synthetic N‐acetyl‐l‐alanine.
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