Occurrence of MBLs and Carbapenemases among MDR and XDR Acinetobacter baumannii Isolated from Hospitals in Iraq

Abstract

The current study was conducted to determine carbapenemase-producing Acinetobacter baumannii clinical isolates recovered from two hospitals in Iraq. A total of 540 different clinical samples were collected. Isolates were identified and Carbapenemase production were detected using MBL E-test, Modified Hodge's Test (MHT), and Modified Carba NP test. A total 30 A. baumannii isolates were obtained from clinical specimens. It was found that 73.3% isolates were carbapenem resistant. Out of 30 A.baumannii isolates, 22 (73.3%) isolates were confirmed to be MBL positive by MBL E-test method, 5(16.6%) by MHT, and 27 (90%) isolates by Modified Carba NP test. Results revealed that 21 A.baumannii isolates (70%) were found to be MDR and only 9 isolates (30%) were XDR. It can conclude that most of carbapenem resistant A.baumannii clinical isolates were MDR. The CNPt assay was the most reliable among phenotypic assays to detect MBL production.