Abstract
White clover (Trifolium repens L.) is a herbaceous, perennial plant that has become one of the most widely distributed legumes in the world. It is cultivated in a large area in China, mainly for animal husbandry feed, artificial grassland, garden greening and ecological construction. In 2022, leaf spot disease was observed on white clover in China's Sichuan province, and the strain was isolated. The pathogenicity of the isolates was confirmed using Koch's postulates. The fungus was/were red on PDA, and two types of conidia were observed after 6 days of cultivation on straw juice medium. The pathogen was identified as Fusarium graminearum by the amplification of part of the ITS and TEF-α genes and by phylogenetic analysis. Currently, this is the first report to indicate that the causal agent of leaf spot disease on white clover is F. graminearum.
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