Abstract
The steroid fraction of hemofiltrate contains a large number of unknown steroids, which were separated by a combination of different chromatographic procedures and finally characterized by gas chromatography mass spectrometry. Besides different isomeric 3,16,17-trihydroxyandrostanes in a rather polar fraction a 3,16,17-trihydroxy-11-ketoandrostane was detected, obviously the first naturally occurring representative of 3,11,16,17-oxygen-tetrasubstituted androstanes.
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