OCCURRENCE OF HELICOBACTER SPECIES WITH SPECIAL PRIORITY TO H. PYLORI IN HEN'S EGGS

Marwa Ezzat Abdel Gaid Kholeaf ,Nagah Saad ,Mostafa Khalil Mostafa ,Marwa Mohammed Nabil Hassan

doi:10.21608/avmj.2018.168944

Marwa Ezzat Abdel Gaid Kholeaf , Nagah Saad + Show 2 more

Open Access

https://doi.org/10.21608/avmj.2018.168944

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Abstract

A total of 300 random eggs, representing 60 samples, (30 from baladi hens, and 30 from poultry farms) were collected from different markets, poultry farms, groceries and supermarkets located in Assuit governorate, Egypt. Each egg sample composed of 5 eggs and these samples were examined for the incidence of Helicobacter species using Helicobacter pylori special peptone agar (HPSPA). The results revealed that the incidence of Helicobacter species from examined baladi hen's egg shells was 10 (33.33%), poultry farms hen's egg shells was 9 (30%) and 1(3.33%) from examined baladi hen's egg content samples. H. pylori was isolated in a percentage of 5 (16.67%) from examined baladi hen's egg shells while from poultry farms hen's egg shells was 4 (13.33%). In addition, otherHelicobacter species were isolated from the examined samples as H. cinaedi in a percentage of (6.67%) from examined baladi hen's egg shells and (10%) from examined poultry farms hen's egg shells. H. felis was isolated in a percentage of (6.67%) from both examined baladi and poultry farms hen's egg shells samples and H. pullorum was isolated in a percentage of (3.33%) from examined baladi hen's egg shells. Also, it wasisolated from examined baladi hen's egg contents in a percentage of (3.33%). Identification of ureC gene for H. pylori isolated from both baladi hen's egg shells and poultry farms hen's egg shells by using polymerase chain reaction (PCR) revealed that 8 from 9 samples were positive for H. pylori by using (PCR) in a percentage of (88.89%).

Highlights

Eggs are among the few foods that are used throughout the world regardless of religion and ethnic groups (Stadelman and Cotterill, 2001)
Results of biochemical identification of Helicobacter species by using conventional method revealed that 9 isolate of samples (15%) out of 20 samples were identified as H. pylori, 5 (16.66%) strains recovered from balady egg shell samples and 4 (13.33%) strains from poultry farms hen's egg shell samples
By using polymerase chain reaction (PCR) technique for identification of suspected isolates, the results revealed that 8 out of 20 samples isolated were identified as H. pylori, where 4 from balady egg shell samples and 4 from poultry farms hen's egg shells samples

Summary

Introduction

Eggs are among the few foods that are used throughout the world regardless of religion and ethnic groups (Stadelman and Cotterill, 2001). Eggs remain a stable food within the human diet, consumed by people throughout the world in various dishes and considered very nutritious and a cheap source of protein (Osei-Somuah et al, 2003). Fecal matter, improper washing, using of contaminated water and bad handling are the common sources of contamination. In spite of their high nutritive value, eggs were responsible for several outbreaks and were a vehicle for transmission of certain human pathogens as Helicobacters (Miyamota et al, 1998; Hangombe et al, 1999; Gast et al, 2004)

Results

Discussion

Conclusion