Abstract
A total of 600 Escherichia coli isolates recovered from pig wastes from three Senatorial Zones of Imo State, Nigeria, were tested for production of extended-spectrum β-lactamases (ESBLs) by the double-disk (DD) potentiation method. Of the numbers of isolates made, 190 (32%) were positive for the ESBLs production. Results of testing 190 positive isolates for ESBL production by several recommended methods were as follows (percentage detection in parentheses): DD method with aztreonam (91), ceftazidime (82), ceftriaxone (84), or cefpodoxime (94); broth microdilution method with ceftazidime (77) or cefotaxime (96) alone or in combination with clavulanate; and the standard disk diffusion method with new breakpoints and standard concentrations of aztreonam (69), ceftazidime (81), ceftriaxone (77), or cefpodoxime (98) or a novel concentration (5 μg) of ceftazidime (84). These data indicate that ESBLs occur at a relatively high incidence in our piggery farms and that the standard disks diffusion method with cefpodoxime and the DD method with several β-lactams are practical and cost-effective methods for detecting ESBL-producing isolates of E. coli.
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