Occurrence of DEA1.1 in blood donor dogs in Cuiabá, Mato Grosso, Brazil

Abstract

This study aimed to assess Dog Erythrocyte Antigen (DEA) 1.1 in donor dogs at the Federal University of Mato Grosso, Cuiabá, Brazil, and review the relevant literature. The blood (60 samples; 1.5 mL volume, each) was collected in separate vacutainer tubes containing ethylenediaminetetraacetic acid and submitted for complete blood count; in addition, the samples were typed by RapidVet® based on agglutination due to specific interaction between DEA 1 antigen at the membrane surface of the erythrocyte and lyophilised murine monoclonal antibody on the test card. DEA1.1 positivity was observed in 81.6% (49 of 60) of test samples, while negative results were obtained in the remaining 18.3% (11 of 60). DEA 1.1 positive samples were comprised of 42.8% of purebred dogs and 38.3% of mixed breed dogs. With regard to sex in the DEA 1.1 positive group, 48.3% were male dogs and 33.3% were female dogs. The blood donor canine population showed high prevalence of DEA 1.1, which confirms that blood typing should be performed prior to blood transfusion in previously sensitised dogs.