Abstract
Objective: To study the prevalence of Coagulase-negative Staphylococci in clinical and subclinical mastitis in dairy cows. Design: Case-control study. Animals: 415 lactating Holstein cows. Procedures: A total of 896 quarter milk samples were collected aseptically from 415 dairy cows for bacteriological examination (mastitic cows, n=50; healthy cows, n=174). Identification of different bacterial isolates followed by antibiotic sensitivity test for Coagulase-negative Staphylococci (CNS) was carried out, using disc-diffusion method against 13 different antibiotics . Results: 224 out of 415 cows (54%) were proven to be mastitic (12% clinical mastitis, 42 % subclinical mastitis). Coagulase-negative Staphylococci were the prevalent isolate (32%). CNS was recorded in 25.7 % of clinical cases and 34% of subclinical mastitis. The antibiogram of CNS isolates against 13 different antibiotics revealed high level of resistance to ampicillin (85%) and oxacillin (85%). However, they were less resistant to vancomycin (5%). The other antibiotics showed variable levels of resistance ranging from 40-75%. Conclusion and clinical relevance: Coagulase-negative Staphylococci were the most common bacteria isolated from mastitic cases. Multi-drug resistance was observed among CNS with high resistance to ampicillin and penicillin, so the use of such B-lactam antibiotics should be restricted specially if there were suspected cases of Coagulasenegative Staphylococci.
Highlights
IntroductionMastitis is one of the most costly diseases of the dairy cattle in Egypt [1]
Coagulase negative Staphylococci were the predominant bacteria isolated from mastitic cases (32%) followed by E. coli (24.8%), S. aureus (15.4%), Str. agalactiae (13.4%), Str .dysgalactiae (8.8%), E. faecalis (2%), Str. uberis (2%), Enterobacter aerogenes (0.66%), Micrococcus (0.66%), Enterobacter agglomerans (0.22%) and Klebsiella spp. (0.22%)
In table (3), the antibiogram of Coagulase-negative Staphylococci isolated from mastitic milk samples was shown
Summary
Mastitis is one of the most costly diseases of the dairy cattle in Egypt [1]. Mastitis adversely decrease both quality and quantity of the milk [2]. Mastitis can be categorized into two forms, which are clinical and subclinical. The clinical form is characterized by one or more of visible signs in the udder as edema, pain, redness, hotness and can be associated with changes in the milk. It may be accompanied with general systemic disorders. The subclinical form has no visible signs so, it is called “the silent form” [3]
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