Occurrence of canine parvovirus in dogs from Henan province of China in 2009-2014.

Zhanqin Zhao,Chunping Peng,Yun Xue,Qiao Xue,Huisheng Liu,Ke Ding,Zuhua Yu

doi:10.1186/s12917-016-0753-1

Zhanqin Zhao, Chunping Peng + Show 5 more

Open Access

https://doi.org/10.1186/s12917-016-0753-1

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Abstract

BackgroundThere is no information concerning the genotype of Canine parvovirus (CPV) currently circulating in Henan province, China. Therefore, the aim of the present study was to provide insights into the epidemiology and molecular characterization of CPV circulating in Henan province from 2009 to 2014.ResultsNineteen thousand nine hundred seven dogs from pet hospitals in the cities of Luoyang, Anyang, Jiaozuo, Sanmenxia, Xinxiang, Zhengzhou in Henan province between 2009 and 2014 were investigated. Over the 6-year period, 1169 CPV-positive cases were identified and the morbidity of CPV infection ranged from 4.16 to 8.06 %, although morbidity was not significant (P > 0.05) between 2009 and 2014. Factors associated with morbidity included sampling season, dog age, breed, vaccination status, and sex. CPV co-infection with coccidium (10.00 %), canine distemper virus (4.79 %), hookworm (2.40 %), canine coronavirus (1.11 %), roundworm (1.03 %), tapeworm (0.17 %) and Babesia spp. (0.09 %) were observed. The new CPV-2a variant was more prevalent than the new CPV-2b variant in Henan province. CPV 2c was not observed in this study.ConclusionsThe epidemiology of CPV infection and identification of the circulating genotypes in Henan province, China from 2009 to 2014 determined that the new CPV-2a variant was more prevalent.

Highlights

There is no information concerning the genotype of Canine parvovirus (CPV) currently circulating in Henan province, China
Faecal samples from suspected CPV-positive dogs were transferred to our laboratory for detection of canine distemper virus (CDV) and canine coronavirus (CCoV) using a CDV or CCoV colloidal gold test strip (Bionote, Inc.), respectively, and for detection of coccidium, ancylostome, roundworm, tapeworm, babesiosis and nematode by microscopy [24] according to the characteristic of these parasites
Morbidity of CPV infection in dogs from Henan province One thousand one hundred seventy eight CPV-positive cases were identified by the CPV colloidal gold test strip

Summary

Introduction

There is no information concerning the genotype of Canine parvovirus (CPV) currently circulating in Henan province, China. Canine parvovirus 2 (CPV-2), a member of the genus Protoparvovirus within the family Parvoviridae (the 2014 ICTV taxonomy, website: http://ictvonline.org/ virusTaxonomy.asp), is a small, highly contagious, nonenveloped icosahedral virus that causes acute gastroenteritis mainly in domestic dogs [1,2,3,4,5]. The full-length VP2 sequence (1755 bp) encodes the major capsid protein, which is 584 aa in length and plays an important role in determining viral host ranges, tissue tropisms, and genetic and antigenic properties [9, 10]. A few years after its emergence, the original virus type CPV-2 was replaced worldwide by three new antigenic variants (i.e., CPV-2a, CPV-2b and CPV-2c) based on aa substitutions (CPV-2a: Val-555-Ile, Asp-305-Tyr, Ala-300-Gly, Ile-101-Thr and Met-87-Leu; CPV-2b: Ile-555-Val and Asp-426-Asn; CPV-2c: Asp-426-Glu) in the VP2 gene [12,13,14,15,16,17,18]

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