Abstract
The genus Campylobacter is one of great importance to public health because it includes several species that may cause diarrhea. Poultry and poultry products are known as important sources of human campylobacteriosis. 225 samples were collected from (75) chickens including intestinal content (75), liver (75) and skin (75). The overall occurrence of Campylobacter jejuni and Campylobacter coli in chicken by PCR were (5.3% & 17.8%). Multiplex PCR targeting 23S rRNA specific for genus Campylobacter, hip O gene specific for C. jejuni and glyA gene specific for C. coli was used for the confirmation of phenotypically identified C. jejuni and C. coli isolates. It is concluded that PCR was determined to be more specific and rapid than biochemical tests.
Highlights
Campylobacter food borne illness incidence is considered the major cause of diarrhea in developed and developing countries
Poultry and poultry products are known as important sources of human campylobacteriosis. 225 samples were collected from (75) chickens including intestinal content (75), liver (75) and skin (75)
The overall occurrence of Campylobacter jejuni and Campylobacter coli in chicken by PCR were (5.3% & 17.8%)
Summary
Campylobacter food borne illness incidence is considered the major cause of diarrhea in developed and developing countries. The high incidence of Campylobacter diarrhea, as well as its duration and possible sequelae, makes it highly important from a socio-economic perspective (World Health Organization, 2011). Campylobacter species are widely distributed in most warm-blooded animals. They are prevalent in food animals such as poultry, cattle, pigs, sheep, ostriches, shellfish and in pets, including cats and dogs (World Health Organization, 2011). In persons with compromised immune systems, Campylobacter occasionally spreads to the blood stream and causes a serious life-threatening infection (Centers for Disease Control and Prevention, 2013). Most Campylobacter associated diarrhea is self-limited, complications can occur.
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