Abstract
Methods In this study we attempted to detect and molecularly characterize viruses infecting Jatropha in Eastern Africa (Kenya and Ethiopia). Detection methods will be valuable tools for early screening of plant viruses in order to make appropriate decisions and selection of planting material. A total of 127 Jatropha samples from Ethiopia and Kenya (districts: Kakamega, Siaya, Busia and Nakuru showing typical virus symptoms and symptomless plants were used in this study. ELISA was performed to detect the presence of three RNA viruses: CMV, Cassava common mosaic virus (CsCMV) and Cassava brown streak virus (CBSV). PCR was performed using newly designed primers based on multiple alignments of full length DNA A sequences of geminiviruses available in the NCBI Genbank, reported to infect either Jatropha or
Highlights
Jatropha curcas is a drought resistant shrub native in tropical America, widely grown in many tropical and subtropical regions for biodiesel production [1]
First reports on virus infections in Jatropha indicated the occurrence of viruses closely related to Cassava mosaic virus in India, reaching a disease incidence from 25 to 47%
None of the Jatropha samples analysed was infected with the RNA viruses CBSV, Cucumber mosaic virus (CMV) and CsCMV
Summary
Jatropha curcas is a drought resistant shrub native in tropical America, widely grown in many tropical and subtropical regions for biodiesel production [1]. First reports on virus infections in Jatropha indicated the occurrence of viruses closely related to Cassava mosaic virus in India, reaching a disease incidence from 25 to 47%. Methods In this study we attempted to detect and molecularly characterize viruses infecting Jatropha in Eastern Africa (Kenya and Ethiopia).
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