Abstract
ABSTRACT The objective of the study was to identify and characterise Fusarium species associated with guava wilt disease. A total of 50 root and soil samples were collected from Guava orchards located in different regions of India. Fusarium species were isolated, identified and categorised into 4 distinct species i.e. Fusarium oxysporum (44.31%), F. solani (36.36%), F. verticillioides (12.5%) & F. chlamydosporum (6.81%) based on the cultural characters and microscopic observations. Translation elongation factor 1-alpha (TEF-1α) gene fragment of 650 bp was sequenced for 32 representative isolates and they were confirmed as F. oxysporum f. sp. psidii, F. verticillioides, F. chlamydosporum & F. falciforme. All four species were tested individually for pathogenicity assays but, only F. oxysporum f. sp. psidii & F. falciforme confirmed with pathogenicity and proved as causal organisms of guava wilt disease in India. F. falciforme is being reported first time in India as a causal organism of guava wilt.
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