Year-end Sale % OFF 
Abstract
The aim of this study was to assess the occurrence of Cryptosporidium in domestic animals in rural properties surrounding rain forest fragments within the municipality of Teodoro Sampaio, southeastern Brazil. Conventional sucrose flotation method followed by molecular characterization of the parasites by sequencing PCR products amplified from SSU rRNA gene were used. Stool samples were collected from domestic animals raised as pets and livestock in all rural properties surrounding three forest fragments. Samples from cattle (197), equine (63), pigs (25), sheep (11), and dogs (28) were collected from 98 rural properties. The frequency of occurrence of Cryptosporidium within each animal species was 3.0% (6/197) among cattle and 10.7% (3/28) among dogs. Cryptosporidium was not detected in stool samples from equine, sheep, and pigs. All sequences obtained from the six samples of calves showed molecular identity with Cryptosporidium andersoni while all sequences from dog samples were similar to C. canis. The frequency of occurrence of Cryptosporidium in these domestic animal species was low. The absence of C. parvum in the present study suggests that the zoonotic cycle of cryptosporidiosis may not be relevant in the region studied. The presence of Cryptosporidium species seldom described in humans may be, otherwise, important for the wild fauna as these animals are a source of infection and dissemination of this protozoan to other animal species. The impact and magnitude of infection by C. andersoni in wild ruminants and C. canis in wild canids have to be assessed in future studies to better understand the actual importance of these species in this region.
Highlights
Pontal do Paranapanema is a region in the westernmost of São Paulo State (INSTITUTO FLORESTAL, 2009) that contains a number of rain forest fragments
All the sequences from calves showed molecular identity with C. andersoni while all sequences from dogs were revealed a similarity to C. canis (Figure 2)
The frequency of occurrence of Cryptosporidium among domestic animals raised in rural properties surrounding the forests in Pontal do Paranapanema was low
Summary
Pontal do Paranapanema is a region in the westernmost of São Paulo State (INSTITUTO FLORESTAL,, 2009) that contains a number of rain forest fragments. This region contains the largest preserved area of Atlantic Forest that still exist inland São Paulo State (Atlantic dry forest). The largest Atlantic dry forest fragment in this region is the Morro do Diabo State Park (MDSP), a vast area of 33,845.33 ha within Pontal do Paranapanema region in the Teodoro Sampaio municipality. The occurrence of Cryptosporidium in animals is a public health problem due to the zoonotic potential of some species of this genus. Due to low host specificity of Cryptosporidium, the domestic fauna may pose the sylvatic fauna at risk of infection, as domestic animals may act as source of infection for wild animals (XIAO et al, 2000)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
