Occurrence and exposure assessment of multiple mycotoxins in dried fruits based on liquid chromatography-tandem mass spectrometry

Abstract

A reliable analytical method based on liquid chromatography-tandem mass spectrometry was developed for simultaneous determination of aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1, aflatoxin G2, ochratoxin A (OTA), deoxynivalenol, T-2 and HT-2 toxin, and zearalenone (ZEA) in various dried fruits. A simple one-step sample extraction without using clean-up cartridges made the established method less labour consuming and less expensive, while optimisation of the several important MS/MS parameters, i.e. the scan time and run segments, ensured its sensitivity and selectivity. After careful validation of the method by determining the linearity (R2>0.99), recovery (77.8-115.9%), precision (relative standard deviation ≤19.5%) and sensitivity (limits of quantification in the range of 0.1-10 μg/kg), a survey of 125 dried fruit samples including 25 pistachios, 28 dried longans, 32 raisins and 40 dried dates randomly collected from different markets in Shanghai, China, was performed. Results revealed that 32.0% of samples were contaminated with different mycotoxins, among which, OTA was the most frequent contaminant with the incidence of 29.6% attaining the concentration levels in the range of 0.4-212.6 μg/kg. ZEA was positively found in 2 pistachio samples with the concentrations of 84.9 μg/kg and 426.9 μg/kg. Trace amounts of AFB1 (0.8 μg/kg) and AFB2 (0.2 μg/kg) were also observed in one pistachio sample and one dried longan sample, respectively. To the best of our knowledge, this is the first report to reveal the real situations of mycotoxin contaminations in various dried fruits in China.