Occurence of thioredoxin m activity in the photosynthetic bacteria Rhodopseudomonas capsulata

Abstract

Thioredoxin capable of activating NADP-Malate dehydrogenase from Spinach has been isolated from a cell free extract of Rhodopseudomonas capsulata. These activating factors, approximately 12,000 Daltons in molecular weight, were partially purified after thermal treatment by using gel filtration, ion-exchange and adsorption chromatographic techniques. The occurence of thioredoxin f activity, though more difficult to obtain, was also evidenced in these bacteria. The present results have been tentatively discussed by a comparison between the thioredoxinspresent in higher plants and bacterial fields.