Occupancy of P 2 purinoceptors with unique properties modulates the function of human platelets

Abstract

This report demonstrates that plateletes possess P 2 purinoceptors with unique properties that distinguish them from the ADP (P 2T) receptor. Extracellular ATP, and its poorly hydrolyzable analogues, inhibit collagen- and U46619 (a thromboxane mimetic)-induced platelet aggregations. Adenosine deaminase was without effect on ATP action while reversing the inhibitory effect of adenosine. A unique aspect of the P 2 receptor is the sensitivity to UTP and CTP and insensitivity to GTP. The rank order of inhibition by βγ-methylene ATP, αβ-methylene ATP > ATP indicates that a P 2x-like receptor is present on the platelet membrane. This conclusion is further supported by the nearly complete desensitization to ATP by pre-exposure of platelets to αβ-methylene-ATP. However, unlike previously described P 2x purinoceptors, the inhibition of platelet aggregation by extracellular ATP appears to result, at least in part, from the ATP-induced increase of intracellular cyclic AMP levels apparently coupled through a G s protein. The combined addition of iloprost (0.14 to 1.39 nM) and ATP (18 μM) or ATP (20–40 μM) and the phosphodiesterase inhibitor theophylline (0.5 to 1 mM) synergistically inhibited platelet aggregation implying a common interactive site with adenylate cyclase. This is further substantiated by the ability of the adenylate cyclase inhibitor, 2′,5′-dideoxyadenosine, to abrogate the inhibitory effects of ATP. The protein kinase A (PKA) inhibitor H1004 blocks ATP inhibition of platelet aggregation while the protein kinase C inhibitor H7 did not. This implies that the generation of cyclic AMP, with the subsequent activation of PKA and phosphorylation of selected proteins is required, in part, for the action of ATP.