OC-090 Correlation between Distribution of HLA Haplotypes and Severity of Mucosal Damages

Abstract

<h3>Introduction</h3> Celiac disease is a chronic intestinal inflammation resulting in different degree of intestinal mucosal damages. The disease occurs in genetically predisposed individuals and associated with human leukocyte antigen (HLA)-DQ2/8 heterodimers. The aim of this study is to evaluate the correlation between distribution of HLA haplotypes and the severity of mucosal damages. <h3>Methods</h3> Fifty nineIranian celiac disease patients were evaluated to predict the HLA-DQA1 and -DQB1 genes, using tagging SNPs method. All patients had positive tTGA and/or EMA antibodies and histology according to Modified Marsh classification (Marsh I-IIIc) by Rostami <i>et al</i> <h3>Results</h3> The result of this study show that 86.4% of cases were carriers of HLA-DQ2 and/or HLA-DQ8 heterodimers, either homozygous or heterozygous. Marsh IIIa lesions were seen in 20 cases, Marsh IIIb in 9 and Marsh IIIc in 12 cases. Marsh IIIa is associated with HLA-DQ2 haplotype in 10 cases, IIIb with 4 and IIIc with 10 cases respectively. Marsh IIIa is correlated with HLA-DQ2.5/2.5 in 4 cases followed by HLA-DQ2.5/DQ8 and HLA-DQ2.5/DQX each in 3 cases respectively. <h3>Conclusion</h3> According to high prevalence of HLA-DQ2 haplotype in Iranian population, we can conclude that there is a strong link between severity of mucosal damages and presence of HLA-DQ 2 haplotype. Celiac disease cases with Marsh III (a-c) carry more HLA risk alleles compared to those with Marsh I-II and this difference were statistically significant for DQ2.5 haplotype distribution (P = 0.0001). <h3>Disclosure of Interest</h3> None Declared