Abstract
Herbicide resistance has been a sought‐after trait for turfgrasses, however attempts to commercialize genetically modified (GM) turfgrasses have been unsuccessful. Sethoxydim (2‐[1‐(ethoxyimino]butyl)‐5‐[2‐(ethylthio)propyl]‐3‐hydroxy‐2‐cyclohexen‐1‐one) is a grass‐specific herbicide, and resistance results from one of two possible single base‐pair (bp) mutations. The most common mutation is an isoleucine (Ile) to leucine (Leu) substitution caused by an A to T mutation at position 1781 of acetyl coenzyme A carboxylase. Research was initiated to develop a novel source of resistance to sethoxydim in seashore paspalum (Paspalum vaginatum Swartz) The objectives of the present study were to develop in vitro selection and regeneration protocols to select for naturally occurring mutations conferring herbicide resistance. A dose response experiment was performed to determine the optimum sethoxydim concentration for selection. Callus was induced from immature inflorescences then plated on callus induction medium containing 10 μM sethoxydim for selection. Green plants were regenerated from resistant callus, the Ile to Leu mutation documented, and expression of herbicide resistance confirmed.
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