Abstract
Abstract Ruscus is an evergreen shrub that offers dark-green glossy foliage used as green additions to bouquets and flower arrangements. One of the most significant ways to obtain new varieties of crop and ornamental plants is to induce mutations by radiation. Gamma radiation is most commonly used to obtain mutants in commercial food as well as feed crops and ornamental plants. In this study, we developed tissue culture methods for Ruscus proliferation from rhizomes to obtain rhizomes clusters. These clusters were subsequently irradiated with Gamma rays to obtain unique phenotypes, such as: elongated narrow phylloclades modified stem symmetry and dwarfed growth habit. Such Ruscus types can contribute to the expansion of the floral industry.
Highlights
Ruscus hypoglossum L. is an evergreen rhizomatous herbaceous perennial of the Asparagaceae Juss family (Chase et al, 2009) with a native range from Italy north to Austria and Slovakia and east to Turkey and Crimea (Halada, 1994).There are few scientific works on Ruscus
Ruscus is indigenous to southwestern Europe, but different species are found in the wild, from Western Europe to Iran as well as in Israel
Buds that were detached from the rhizome segments and segments of phylloclade with or without a flower were placed on MS (Murashige and Skoog salt medium) media differing in their plant growth regulators content
Summary
Ruscus hypoglossum L. is an evergreen rhizomatous herbaceous perennial of the Asparagaceae Juss family (Chase et al, 2009) with a native range from Italy north to Austria and Slovakia and east to Turkey and Crimea (Halada, 1994).There are few scientific works on Ruscus. Ruscus is indigenous to southwestern Europe, but different species are found in the wild, from Western Europe to Iran as well as in Israel. Non-photosynthetic spikes, located at the center of the phylloclades, where the very small white flower develops on each phylloclade if conditions are favorable and a red fruit forms (Stamps, 1997). Fruits are red berries and fruiting is rare in this plant. Propagation of Ruscus plants is done by dividing the plant rhizomes into several small clumps that carry several branches and thick roots (Winarto, 2017). Ruscus plants exhibits high adaptability to diverse growing conditions (i.e. light, temperature, and various soil compositions) and has been widely cultivated in public areas of the Middle East since the 12th century (Veroese, 2015)
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